学历：博士研究生

学科：分子影像技术介导的外科手术导航

电子邮件：zhangguojun@kmmu.edu.cn

通讯地址：云南省昆明市西山区昆州路519号

简历：

从事乳腺癌等恶性肿瘤的基础及临床研究30余年，在分子影像技术介导的精准外科和抗肿瘤药物疗效评估等方向取得重要研究成果。建立了以光学分子影像技术为中心的乳腺癌精准诊治技术体系。

经历：

2023.08至今 昆明医科大学第三附属医院，院长

2023.01- 2023.07 厦门大学附属翔安医院，乳甲外科主任

2017.05 - 2022.12 厦门大学附属翔安医院，执行院长

2008.08 - 2017.04 汕头大学医学院附属肿瘤医院，院长；乳腺中心，主任

2005.01 - 2008.07 美国Merck & Co.公司Merck研究实验室，高级科学家

1999.08 - 2004.12 美国Harvard Medical School/Dana-Farber癌症研究所， Howard Hughes医学研究所，博士后研究员

1993.05 - 1995.03 日本国立癌症中心，研修

1987.07 - 1993.04 中国医科大学附属第一医院普外三科，历任住院医师、主治医师、讲师

研究方向：

本方向旨在解决术中难以实时可视化肿瘤边界、评估淋巴结转移状态以及实时保护正常组织的完整性等问题，基于开发靶向肿瘤细胞高表达、肿瘤微环境特异性标志物、转移淋巴结以及正常组织（如输尿管、神经、淋巴管）的 NIR-II 荧光分子探针，推进探针临床转化，通过高灵敏度、高特异性的深层穿透与高分辨率成像，构建术中分子影像导航体系，实现对肿瘤边界的实时动态勾勒、转移淋巴结可视化及对关键组织实时保护不受损伤，减少术后并发症。

代表性成果和所获奖项：

论文：

1.Xue-Qi Fan*, Jing-Wen Bai*, Shi-Long Yu*, Xiao Shen, Lei Niu, Wen-He Huang, Gui-Mei Wang, Zhi-Cheng Du, Xue Zhao, Fang-Hong Zhang, Chen-Hui Yang#, Guo-Jun Zhang#, Intraoperative evaluation of metastatic SLNs with NIRF imaging assisted by artificial intelligence in breast cancers, International Journal of Surgery, 2025 Oct 10.

2.Niu L*, Lou KL*, Zhu Z*, Liu WZ*, Gao WL, Hu GY, Gao JX, Zhang GJ#, Huang WH#, Aptamer-based NIR II imaging for breast cancer surgical resection, Npj Imaging, 2025 Aug 19;3(1):38.

3.Rong Guo*, Xiting Wang*, Yinju Yang*, Jiaying Zou*, Ming Li, Zeying Li, Yuan Yan, Nan Lan, JianYun Nie#, Yiyin Tang#, Guojun Zhang#, Prognostic value of a lncRNA signature in early-stage invasive breast cancer patients, Cancer cell international, 2025 Jun 24.

4.YY He*, JY Lin*, JW Bai*, X Shen, KL Lou, YY Zhu, ZS Qiao, WL Chen, Y Li#, XL Liu#, GJ Zhang#, A Hf(IV)-Coordinated NIR-II Fluorescence "Turn-On" Nanoprobe for Precise Imaging-Guided Surgery in Breast Cancer, Advanced Science, 2025 Feb 14.

5.ZC Du*, FY Fan*, J Ma, J Liu, X Yan, XX Chen, YF Dong, JP Wu, WZ Ding, QX Zhao, YL Wang, GJ Zhang#, J Yu#, P Liang#, Development and validation of an ultrasound-based interpretable machine learning model for the classification of ≤3 cm hepatocellular carcinoma: a multicentre retrospective diagnostic study, EClinicalMedicine, 2025 Feb 13.

6.HY Chen*，LL Lin*, ZS Qiao，YF Pei，YY Gao，KL Lou，LL Yang，CX Li，YY He，JW Bai#，GJ Zhang#, YTHDF3 drives tumor growth and metastasis by recruiting eIF4B to promote Notch2 translation in breast cancer, CANCER LETTERS, 2025 Feb 7.

7.Xue Zhao*, Jing-Wen Bai*, Sen Jiang*, Zhen-Hui Li*, Jie-Zhou He, Zhi-Cheng Du, Xue-Qi Fan, Shao-Zi Li, Guo-Jun Zhang#, Multiparametric MRI and transfer learning for  predicting positive margins in breast-conserving surgery: a multi-center study, INTERNATIONAL JOURNAL OF SURGERY, 2025 Feb 4

8.Chen WL*, Zhang YQ*, Zhang LX*, Luo YX, Yang X, Zhu YY, Wang GM, Huang WH, Zhang DL, Zeng YZ, Ronghui Li, Guo CP, Wang JZ, Zhao W, Liu N, Zhang GJ#. Intraoperative evaluation of tumor margins using a TROP2 near-infrared imaging probe to enable human breast-conserving surgery, Science Translational Medicine, 2024 Oct 16.

9.Ming ZH*, Zhang YQ*, Song L, Chen M, Lin LL, He YY, Liu WL, Zhu YY, Zhang Y, Zhang GJ#. Rare Earth Nanoprobes for Targeted Delineation of Triple Negative Breast Cancer and Enhancement of Radioimmunotherapy. Advanced Science, 2024 May 22.

10.Dang YY*, Bai JW*, Lou KL, Yang RQ, Gao YY, Tian HA, Li JQ, Lin LL, Lv CR, Wang PY#, Zhang GJ#. Intraoperative Surgical Margin Assessment by NIR-II Imaging with Urine Excretable Nd-Based Nanoprobe in Breast Cancers. Adv. Funct. Mater. 2024, https://doi.org/10.1002/adfm.202311673.

11.Li JW*, Deng QM*, Zhu JL, Wei M, Hu XY, Chen HY, Luo Z, Lin LL, Wei XL, Zhang YQ, Lou KL, Gao YY, Zhang GJ#, Bai JW#. Methylation of ESR1 promoter induced by SNAI2–DNMT3B complex promotes epithelial–mesenchymal transition and correlates with poor prognosis in ERα-positive breast cancers. MedComm, 2023 Oct 24;4(6): e403.

12.Liu WL*, Zhang YQ*, Luo XY, Zhu YY, Song L, Ming ZH, Zhang LX, Li MI, Rui-Chan Lv, Zhang GJ#, Chen M#. Novel Dual-Mode NIR-II/MRI Nanoprobe Targeting PD-L1 Accurately Evaluates the Efficacy of Immunotherapy for Triple-Negative Breast Cancer. Int J Nanomedicine. 2023 Sep 8:18:5141-5157.

13.Lin B, Dai RY, Liu ZH, Li WJ, Bai JW, Zhang GJ#, Lv RC#. Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation. J Photochem Photobiol B. 2023 Aug; 245:112731.

14.Zhu YY*, Song L*, Zhang YQ, Liu WL, Chen WL, Gao WL, Zhang LX, Wang JZ, Ming ZH, Zhang Y#, Zhang GJ#. Development of a rare earth nanoprobe enables in vivo real-time detection of sentinel lymph node metastasis of breast cancer using NIR-IIb imaging. Cancer Res. 2023 Aug 4: CAN-22-3432.

15.Qi YZ, Li MF, Li SZ, Zeng D, Xiao YS, Li JW, Ye QQ, Bremer E, Zhang GJ#. Notch1 promotes resistance to cisplatin by up-regulating Ecto-5'-nucleotidase (CD73) in triple-negative breast cancer cells. Cell Death Discovery. 2023 Jun 30;9(1):204.

16.Wei M, Bai JW, Shen X, Lou KL, Gao YY, Lv RC, Wang PY#, Liu XL#, Zhang GJ#. Glutathione-Exhausting Nanoprobes for NIR-II Fluorescence Imaging-Guided Surgery and Boosting Radiation Therapy Efficacy via Ferroptosis in Breast Cancer. ACS Nano. 2023 Jun 27;17(12):11345-11361.

17.Zhang YQ*, Liu WL*, Luo XJ, Shi JP, Zeng YZ, Chen WL, Huang WH, Zhu YY, Gao WL, Li RH, Ming ZH, Zhang LX, Yang RQ, Wang JZ, Zhang GJ#. Novel Self-Assembled Multifunctional Nanoprobes for Second-Near-Infrared-Fluorescence-Image-Guided Breast Cancer Surgery and Enhanced Radiotherapy Efficacy. Adv Sci (Weinh). 2023 Apr;10(10): e2205294.

18.Zhao X*, Bai JW*, Guo Q, Ren K#, Zhang GJ#. Clinical applications of deep learning in breast MRI. Biochim Biophys Acta Rev Cancer. 2023 Mar;1878(2):188864.

19.Bai JW*, Qiu SQ*, Zhang GJ#. Molecular and functional imaging in cancer-targeted therapy: current applications and future directions. Signal Transduct Target Ther. 2023 Feb 27;8(1):89.

20.Chen WL*, Wang GM*, Zhang GJ#. Insights into the transition of ductal carcinoma in situ to invasive ductal carcinoma: morphology, molecular portraits, and the tumor microenvironment. Cancer Biol Med. 2022 Nov 1;19(10):1487–95.

21.Chen WL*, Zhang YQ*, Li RH, Huang WH, Wei XL, Zeng D, Liang YK, Zeng YZ, Chen M, Zhang LX, Gao WL, Zhu YY, Li YC#, Zhang GJ#. Notch3 Transactivates Glycogen Synthase Kinase-3-Beta and Inhibits Epithelial-to-Mesenchymal Transition in Breast Cancer Cells. Cells. 2022 Sep 14;11(18):2872.

22.Liang YK, Voshart D, Paridaen JTML, Oosterhof N, Liang D, Thiruvalluvan A, Zuhorn IS, den Dunnen WFA, Zhang GJ, Lin HY, Barazzuol L, Kruyt FAE#. CD146 increases stemness and aggressiveness in glioblastoma and activates YAP signaling. Cell Mol Life Sci. 2022 Jul 5;79(8):398.

23.Liu WL*, Zhang YQ*, Li LT, Zhu YY, Ming ZH, Chen WL, Yang RQ, Li RH, Chen M#, Zhang GJ#. Application of molecular imaging in immune checkpoints therapy: From response assessment to prognosis prediction. Crit Rev Oncol Hematol. 2022 Jun 22; 176:103746.

24.Li JW, Wu J, Long QY, Wu YA, Hu XY, He YK, Jiang MZ, Li J, Zhao LL, Yang SQ, Chen XY, Wang MH, Zheng JS, Wu FF, Wu RL, Ren LL, Bu L, Wang HZ, Li K, Fu LJ, Zhang GJ#, Zheng YL#,Gao ZC#. Comprehensive Humoral and Cellular Immune Responses to SARS-CoV-2 Variants in Diverse Chinese Population. Research (Wash D C). 2022 Jun 16; 2022:9873831.

25.Gao YY*, Yang RQ*, Lou KL, Dang YY, Dong YY, He YY, Huang WH, Chen M, Zhang GJ#. In vivo visualization of fluorescence reflecting CDK4 activity in a breast cancer mouse model. MedComm (2020). 2022 Jun 10;3(3): e136.

26.Lou KL*, Wang PY*, Yang RQ, Gao YY, Tian HN, Dang YY, Li Y, Huang WH, Min-Chen, Liu XL#, Zhang GJ#. Fabrication of tumor targeting rare-earth nanocrystals for real-time NIR-IIb fluorescence imaging-guided breast cancer precise surgery. Nanomedicine. 2022 Apr 4:102555.

27.Yang RQ*, Chen M*, Zhang Q, Gao YY, Lou KL, Lin TT, Huang WH, Zeng YZ, Zhang YQ, Dang YY, Ren L#, Zhang GJ#. Development and Preclinical Evaluation of a Near-Infrared Fluorescence Probe Based on Tailored Hepatitis B Core Particles for Imaging-Guided Surgery in Breast Cancer. International Journal of Nanomedicine. 2022 Mar 22; 17:1343-1360.

28.Yang RQ*, Wang PY*, Lou KL, Dang YY, Tian HN, Li Y, Gao YY, Huang WH, Zhang YQ, Liu XL#, Zhang GJ#. Biodegradable Nanoprobe for NIR-II Fluorescence Image-Guided Surgery and Enhanced Breast Cancer Radiotherapy Efficacy. Advance Science. 2022 Feb 16: e2104728.

29.Liu JJ*, Wang Z*, Nie LM, Zhu YY, Li G, Lin LL, Chen M#, Zhang GJ#. RGD-functionalised melanin nanoparticles for intraoperative photoacoustic imaging-guided breast cancer surgery. European Journal Nuclear Medicine and Molecular Imaging. 2022 Feb;49(3):847-860.

30.Ouyang YX*, Feng J*, Wang Z, Zhang GJ#, Chen M#. miR-221/222 sponge abrogates tamoxifen resistance in ER-positive breast cancer cells through restoring the expression of ERα. Mol Biomed. 2021 Jun 30;2(1):20.

31.Zhang YQ, Zhang F, Zeng YZ, Chen M, Huang WH, Wu JD, Chen WL, Gao WL, Bai JW, Yang RQ, Zeng HC, Wei XL#, Zhang GJ#. Mutant p53 and Twist1 Co-expression Predicts Poor Prognosis and Is an Independent Prognostic Factor in Breast Cancer. Frontiers in Oncology. 2021 Jun 24; 11:628814.

32.Zhang YQ, Liang YK, Wu Y, Chen M, Chen WL, Li RH, Zeng YZ, Huang WH, Wu JD, Zeng D, Gao WL, Chen CF, Lin HY, Yang RQ, Zhu JW, Liu WL, Bai JW, Wei M, Wei XL#, Zhang GJ#. Notch3 inhibits cell proliferation and tumorigenesis and predicts better prognosis in breast cancer through transactivating PTEN. Cell Death & Disease. 2021 May 18;12(6):502.

33.Yang RQ*, Lou KL*, Wang PY, Gao YY, Zhang YQ, Chen M, Huang WH, Zhang GJ#. Surgical Navigation for Malignancies Guided by Near-Infrared-II Fluorescence Imaging. Small Methods. 2021 Mar;5(3): e2001066.

34.Wang Z, Chen M, Liu JJ, Chen RH, Yu Q, Wang GM, Nie LM#, Huang WH#, Zhang GJ#. Human Serum Albumin Decorated Indocyanine Green Improves Fluorescence-Guided Resection of Residual Lesions of Breast Cancer in Mice. Frontiers in Oncology. 2021 Mar 8; 11:614050.

35.Guo CP*, Guo YX*, Liu JJ, Gao YY, Wei M, Zhao RJ, Chen M#, Zhang GJ#. The G1 phase optical reporter serves as a sensor of CDK4/6 inhibition in vivo. International Journal of Biological Sciences. 2021 Jan 31;17(3):728-741.

36.Wei M*, Bai JW*, Niu L, Zhang YQ, Chen HY, Zhang GJ#. The Complex Roles and Therapeutic Implications of m (6)A Modifications in Breast Cancer. Frontiers in cell and developmental biology. 2021 Jan 11; 8:615071.

37.Wu Y, Yuan MH, Wu HT, Chen WJ, Zhang ML, Ye QQ, Liu J#, Zhang GJ#. MicroRNA-488 inhibits proliferation and motility of tumor cells via downregulating FSCN1, modulated by Notch3 in breast carcinomas. Cell Death & Disease. 2020 Oct 24;11(10):912.

38.Wu JD*, Wang Z*, Zeng HC, He LF, Zhang YQ, Huang GS, Zhang F, Wei XL, Huang WH, Zhang GJ#. Comparison of indocyanine green and methylene blue use for axillary reverse mapping during axillary lymphnode dissection. MedComm. 2020 Sep 17;1(2):211-218.

39.Fang CB, Wu HT, Zhang ML, Liu J#, Zhang GJ#. Fanconi anemia pathway: mechanisms of breast cancer predisposition development and potential therapeutic targets. Frontiers in Cell and Developmental Biology. 2020 Apr 2; 8:160.

40.Bai JW*, Wei M*, Li JW, Zhang GJ#. Notch signaling pathway and endocrine resistance in breast cancer. Frontiers in Pharmacology. 2020 Jun 19; 11:924.

41.Zhang YQ, Chen WL, Zhang F, Wei XL, Zeng D, Liang YK, Wu JD, Zhang LY, Guo CP, Zeng HC, Hao SS, Li RH, Huang WH#, Zhang GJ#. Over expression of both VEGF C and twist predicts poor prognosis in human breast cancer. Clinical and Translational Oncology. 2019 Sep;21(9):1250-1259.

42.Li MF, Qi YZ, Chen M, Wang Z, Zeng D, Xiao YS, Li SZ, Lin HY, Wei XL, Zhang GJ#. GATA binding protein 3 boosts extracellular ATP hydrolysis and inhibits metastasis of breast cancer by up-regulating ectonucleoside triphosphate diphosphohydrolase 3. International Journal of Biological Sciences. 2019 Sep 7;15(12):2522-2537.

43.Chen CF, Zhang YL, Huang ZY, Wu JD, Huang WH#, Zhang GJ#. Decrease in the Ki67 index during neoadjuvant chemotherapy predicts favorable relapse-free survival in patients with locally advanced breast cancer. Cancer Biology & Medicine. 2019 Aug;16(3):575-586.

44.Zeng HC, Hu JL, Bai JW, Zhang GJ#. Detection of sentinel lymph nodes with near-infrared imaging in malignancies. Molecular Imaging and Biology. 2019 Apr;21(2):219-227.

45.Xu JZ*#, Shao CC*, Wang XJ, Zhao X, Chen JQ, Ouyang YX, Feng J, Zhang F, Huang WH, Ying Q, Chen CF, Wei XL, Dong HY, Zhang GJ#, Chen M#. circTADA2As suppress breast cancer progression and metastasis via targeting miR-203a-3p/SOCS3 axis. Cell Death & Disease. 2019 Feb 20;10(3):175.

46.Xiao YS, Zeng D, Liang YK, Wu Y, Li MF, Qi YZ, Wei XL, Huang WH, Chen M, Zhang GJ#. Major vault protein is a direct target of Notch1 signaling and contributes to chemoresistance in triple-negative breast cancer cells. Cancer Letters. 2019 Jan;440-441:156-167.

47.Shen JX, Liu J#, Zhang GJ#. Interleukin-33 in malignancies: friends or foes? Frontiers in Immunology. 2018 Dec 20; 9:3051.

48.Li RH, Chen M, Liu J, Shao CC, Guo CP, Wei XL, Li YC, Huang WH, Zhang GJ#. Long noncoding RNA ATB promotes the epithelial-mesenchymal transition by upregulating the miR-200c/Twist1 axe and predicts poor prognosis in breast cancer. Cell Death & Disease. 2018 Dec 5;9(12):1171.

49.Wen XF*, Chen M*, Wu Y, Chen MN, Glogowska A, Klonisch T#, Zhang GJ#. Inhibitor of DNA binding 2 inhibits epithelial-mesenchymal transition via up-regulation of Notch3 in breast cancer. Translational Oncology. 2018 Oct;11(5):1259-1270.

50.Liu J, Shen JX, He, Zhang GJ#. Bioluminescence imaging for monitoring miR-200c expression in breast cancer cells and its effects on epithelial-mesenchymal transition progress in living animals. Molecular Imaging and Biology. 2018 Oct;20(5):761-770.

专利：

1.一种辅助固定麻醉鼠成像的小鼠恒稳仪（实用新型专利；专利号：ZL202422599661.2；发明人：张国君、陈俊宏、陈伟玲、朱媛媛）

2.一种镧系稀土纳米探针及其制备方法和应用（发明专利；专利号：ZL202310679769.X；发明人：张国君、明梓何、宋良、张永渠、黄文河、白静雯、林琳玲、何悦洋）

3.一种NIR-Ⅱ发射的稀土纳米探针及其制备方法和应用（发明专利；专利号：ZL202310058047.2；发明人：张国君、刘小龙、党永颖、王培园、白静雯、楼康良、黄文河）

4.一种用于肿瘤 NIR-Ⅱ荧光成像及放疗增敏-免疫联合治疗的可降解分子探针及应用（发明专利；专利号：ZL202410485430.0；发明人：张国君、何悦洋、李阳、白静雯、曹艳兵、沈筱）

5.一种靶向 Trop-2 的近红外二区荧光探针及其制备方法和应用（发明专利；专利号：ZL202211668918.4；发明人：张国君、陈伟玲、张莉欣、张永渠、朱媛媛、黄文河、王佳铮）

6.一种引导乳腺癌保乳切缘精准评估的荧光/核磁探针（发明专利；专利号：ZL 202210234057.2 ；发明人：张国君、张永渠、刘婉玲、Min Chen、罗祥杰、黄文河）

7.一种用于无创评估乳腺癌前哨淋巴结转移的靶向分子探针（发明专利；专利号：ZL 202210234060.4 ；发明人：张国君、朱媛媛、宋良、张永渠）

8.一种用于乳腺癌NIR-II区荧光手术导航及放疗增敏的靶向分子探针（发明专利；专利号：ZL 2022 1 1210287.1；发明人：张国君、魏敏、王培园、白静雯、黄文河、高艺洋）

9.活体内检测CK4/6激酶活性的分子探针及其应用（发明专利；专利号：ZL 2021 1 1294233.3；发明人：张国君、高艺洋、杨瑞钦、董媛媛、Min Chen、楼康良）

10.基于超声医学的乳腺结节检测方法及系统（发明专利；专利号：ZL202010924386.0；发明人：张国君、李卫斌、陈敏、王连生、陈云超、徐辉雄）

11.一种用于引导肿瘤切除的光声成像手术导航平台（发明专利；专利号：ZL202010645805.7；发明人：张国君、王尊、陈敏、聂立铭、黄珊珊、李宏辉）

12.赵旭晔、张国君、黄文河、康欣梅、牛蕾、李良涛（实用新型；专利号：ZL202021711459.X；发明人：赵旭晔、张国君、黄文河、康欣梅、牛蕾、李良涛）

13.hTERT-miR-221/222海绵及其在逆转乳腺癌的他莫昔芬耐药中的应用（发明专利；专利号：ZL201910364686.5；发明人：张国君、陈敏、欧阳檐秀、冯军）

细胞周期S期报告基因（发明专利；专利号：ZL201110075260.1；发明人：张国君、赵瑞君、陈志鸿）

获奖：

1.中华医学会乳腺肿瘤学组 “2024年度乳腺领域十大原创研究”(项目名称：乳腺癌术中肿瘤切缘精准评估分子影像技术；完成人：张国君，陈伟玲，张莉欣，张永渠，朱媛媛，黄文河，王佳铮)

2.2021年度福建省科学技术进步奖三等奖（项目名称：创新光学分子影像技术体系的建立及其在乳腺癌精准外科的应用；完成人：张国君，黄文河，白静雯，邱斯奇，曾焕城）

3.厦门市2020-2021年度十大医疗创新技术（项目名称：乳腺癌精准外科中分子影像技术的创新研究与应用；完成人：张国君，黄文河，白静雯，陈伟玲等）

4.2020年度中国抗癌协会科技奖二等奖（项目名称：分子影像导引的乳腺癌诊疗一体化研究及临床应用；完成人：张国君，迟崇巍，王殊，Min Chen，杜彩文，白静雯，梁元科）

5.2020年厦门市科学技术进步奖二等奖（项目名称：可视化分子影像技术在乳腺癌精准外科的基础研究与临床应用；完成人：张国君，刘静，迟崇巍，黄文河，杜彩文，白静雯，陈志鸿，李荣晖）

6.2019年度辽宁省科学技术进步奖二等奖（项目名称：乳腺癌多学科综合治疗策略的建立与应用；完成人：滕月娥，郭志刚，张国君，刘云鹏，徐璐，陈波，米小轶，张立娜，石晶）

7.2016年度广东省科学技术奖三等奖（项目名称：乳腺癌转移进展机制及预后研究；完成人：张国君，杜彩文，刘静，崔玉坤，黄文河，魏晓龙，陈宏武）

8.第九届潮汕星河国瑞科技奖（完成人：张国君）

9.2014年度汕头市科技进步奖一等奖（项目名称：乳腺癌转移进展机制及预后研究；完成人：张国君，杜彩文，刘静，崔玉坤，黄文河，魏晓龙，陈宏武，许宏武，马长春）

承担课题：